Answer to Question #11763 in Microbiology for Marcy Randall
Bacteria that use the mixed acid fermentation pathway produce high levels of organic acid—such high levels of acid causes the methyl red to turn red in color. If the amount of acid production is relatively lower, then the medium remains yellow or turns slightly orange after the addition of methyl red. If the media remains red this is a positive (+) reaction for the MR test if the medium remains yellow or turns slightly orange, this is a negative reaction for the MR test.
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