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In certain cats, tail can be long, manx (no tail), or in the case of the heterozygous condition, short. What is the probability that many offspring are produced if a short tailed cat is bred with a manx cat?v
You order a PCR primer from Eurofins Genomics. They deliver a tube with 35 nmoles of DNA
in it. How much TE buffer do you need to turn it into a 100 µM freezer stock? Which pipettor
should you use? To what should it be set?
An individual has the following genotype: AABbDdeeFfGghh. How many unique gametes can
they form?
Recombinase is a protein that finds adjacent pieces of DNA that have nearly identical DNA sequences, facilitates a backbone break in each piece of DNA near the identical sequence, physically rearranges the pieces of DNA, and then facilitates backbone repair. How does recombinase know when it’s found the right combination of DNA?
Huntington’s disease is caused by an allele at the IT15 gene. The allele produces RNA leading to
a protein that has a novel impact on how it interacts with other proteins, causing
neurodegenerative action. Is this allele best classified as functional, non-functional, or
abberant? Justify your answer in 1-2 sentences.
Unlike eukaryotic cells, prokaryotic cells do not have to remove introns and splice together exons to make a functional mRNA molecule.

Which type of cell has an obvious advantage in terms of energy cost to synthesize proteins? Which type of cell has a greater opportunity to control gene expression? Explain.
What is the advantage of altering the protein that is made, instead of just stopping protein production?
Why is it important for gene regulation to be reversible in a cell?
If a wildtype bacterium is grown in the presence of the sugar X-gal, transcription of the polycistronic m-RNA does not occur. What is X-gal unable to do? Without adding lactose (or genetically modifying the bacteria), how could you fix this problem
If a lacI - bacterium is grown in the presence of the sugar X-gal, a blue dye appears; however, the concentration of X-gal decreases over time. What has happened to the X-gal?
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