Answer to Question #75315 in Cell Biology for Shahid ul islam
Salting out separates molecules on the basis of solubility (macromolecules interact either with positively or negatively charged stationary phase).
Affinity chromatography separates molecules on the basis of molecular affinity (chromatographic column is set up for which the stationary phase contains a functional group that interacts specifically with the protein of interest).
Electrophoresis that separates molecules on the basis of charge, the charge is transmitted via the ions
provided by the buffer.
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