Answer to Question #73715 in Cell Biology for Jagadish kalita
molecular size, charge, solubility and molecular affinity.
Separation based on charge is performed with the help of ion exchange chromatography. Charged resin either attracts the particles or contributes to their faster passage through the tube. For more information visit https://www.biochemden.com/ion-exchange-chromatography/
Separation based on solubility is performed with the help of paper chromatography, when molecules move along with solvent according to their ability to interact with it. For more information see https://www.chemguide.co.uk/analysis/chromatography/paper.html
Separation by molecular affinity is performed with the help of affinity chromatography, when specific antibodies are used in the column. See for more information https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-affinity-purification.html
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