Answer to Question #171299 in Cell Biology for Cameron Hicks

Question #171299

I am looking at studying depolarization in catfish, using a cell culture I hope to measure this using muscle cells taken from the swimming muscle. I am also looking into the effect of a cholinergic crisis on depolarization. My question is, would I be able to sustain these cells under a cholinergic crisis, if I provided the necessary nutrients (Could this be explained if not)? If not, what causes damage to the depolarization function of the muscle cell during this crisis?

Many thanks

Expert's answer


Depolarization of cone catfish horizontal cells induces an extracellular. If a stimulus reaches or exceeds the threshold potential of the cardiac muscle cell (about -70 mV), phase 0 takes place. This is known as the depolarization period and is caused by the opening of the sodium voltage-gated time-dependent channels and this allows sodium ions into the cell.

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