Answer to Question #123361 in Cell Biology for AAKASH DEEP

Question #123361
The initial concentration of cell culture is 500 cells per mL. After 3 hours, the of cells in one mL is 2,56,000. calculate the generation time of cells
1
Expert's answer
2020-06-22T13:29:49-0400

The generation time is the time interval required for the cells (or population) to divide.

G (generation time) =t (time, in minutes)/n(number of generations)

G = t/n

t = time interval in minutes = 180 minutes

B = number of bacteria at the beginning of a time interval = 500 cells 

b = number of bacteria at the end of the time interval = 2,56,000 cells

n = number of generations (number of times the cell population doubles during the time interval)

b = B x 2n (This equation is an expression of growth by binary fission)

Solve for n:

logb = logB + nlog2

n = logb - logB

          log2

n = logb - logB

          .301

n = 3.3 logb/B

G = t/n

Solve for G

G =       t

      3.3 log b/B

G =       180

      3.3 log 256000 –log500

=180 / 8.943

G = 20.12 minutes per generation


 


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Comments

AAKASH DEEP
20.06.20, 17:25

Which of the following is TRUE as per Darwinian evolutionary theory? 1. Variations between members of a species is significantly important for evolution. 2. Aggressive animals have better chances to survive, in competition for survival. 3. Offsprings may inherit advantageous behaviour attained during the life span of their parent. 4. Species continue to evolve if they have adapted perfectly to a stable environment.

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