Answer to Question #47590 in Bioinformatics for Sherouk Youssef
However, in Bradford method, you measure absorbance at 595 nm. As NaCl does not absorb at this wavelength, you could use water as control. The only reason to use 0.15M NaCl (or any dilution buffer) as a control is just technical – to be sure that your buffer does not contain any substances that could interact with Bradford reagent and give false positive signal.
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