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Answer to Question #86072 in Biochemistry for Ankur Solanki

Question #86072
Give the role of following vitamins in biochemical reactions.Explain with the help of an example.
(i)Thiamine
(ii) Pyridoxine
(iii)Riboflavin
Expert's answer

1). The derivative of thiamine, thiamine pyrophosphate (TPP) is a coenzyme for several enzymes that catalyze the transfer of two-carbon units and in particular the dehydrogenation of alpha-keto acids. For example TPP is a coenzyme of pyruvate dehydrogenase and responsible for the transfer of pyruvate to acetyl-CoA by attacking the electrophilic ketone of pyruvate.

2). The derivative of pyridoxine - pyridoxal phosphate acts as coenzyme in such biochemical reaction as decarboxylation, for example conversion of 5-hydroxytryptophan (5-HTP) to serotonin.

3). Riboflavin forms two coenzymes - flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). FMN is a cofactor of oxidoreductases in electron transport chain. FMN is a strong oxidizing agent because it can take part in both one- and two-electron transfers. FAD is a coenzyme of various aerobic dehydrogenases (for example, α-ketoglutarate dehydrogenase and a component of the pyruvate dehydrogenase complex).FAD acts as an acceptor of H and e in its fully oxidized form, an acceptor or donor in the FADH form, and a donor in the reduced FADH2

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