Answer to Question #83754 in Biochemistry for Effah George
phosphate to form fructose 1,6-bisphosphate and ADP and discovered that a plot of the
initial velocity of formation of fructose 1,6-bisphosphate versus ATP concentration was
not hyperbolic, but rather sigmoid, what would you suspect?
Sigmoidal velocity of formation of the end product can be explained by high concentration of ATP.
ATP causes this effect by binding to a specific regulatory site of enzyme, which is distinct from the regulatory site. Hence the high concentration of ATP has an inhibitory effect on velocity of reaction.