Answer to Question #69185 in Biochemistry for Alex Ru

Question #69185
The following data about a protein sequence was collected. You know that the peptide is 37 amino acids long. 1) cleavage with CNBr (using the single letter amino acid code): M1: I-P-G-W-V-M M2: S-K-K-G-G-N-I-P-M M3: D-F-P-T-G-K-E-S-G-D-F-L 2) Cleavage with trypsin: T1: S-K T2: E-S-G-D-F-L-A-I-D-L-G T3: G-G-N-I-P-M-I-P-G-W-V-M-D 3) cleavage with chymotrypsin: C1: L-A-I-D-L-G-G-T-N-L-R C2: S-K-K-G-G-N-I-P-M-I-P-G-W C3: V-M-D-F-P-T-G-K-E-S-G-D-F 4) cleavage with Endopeptidase Asp-N (“Endo Asp”): EA1: D-F-P-T-G-K-E-S-G EA2: S-K-K-G-G-N-I-P-M-I-P EA3: D-F-L-A-I- EA4: D-L-G-G-T-N-L-R How was this data generated? You can show this in a flow chart.
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