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Answer to Question #68169 in Biochemistry for Brendan

Question #68169
You are attempting to determine Km be measuring the reaction velocity at different substrate concentrations, but you do not realize that the substrate tends to precipitate under the experimental conditions you have chosen. How would this affect your measurement of Km?
Expert's answer
The experimentally determined Km will be greater than the true Km. This is because the actual substrate concentration is less than expected.

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