Answer on Biochemistry Question for ivan
Arg (10.5), Lys (12.9), His (6.1), Asp (3.9), Glu (4.3).
At the pI the net charge of the molecule is zero.
At 9.93 Lys (pKa=12.9) and Arg (pKa=10.5) are charged positively, His has zero charge (pKa=6.1). Thus, overall positive charge is 20+15=35. Thus, ideally we need 35 Asp/Glu to compensate this positive charge, and the protein will have pI of 9.93.
Comment: real pI of this protein would be less, so in fact we need less Asp/Glu to compensate the positive charge. pI also depends on pKa values used, and folding of the protein also affects its pI.
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