63 776
Assignments Done
99,2%
Successfully Done
In August 2018

Answer to Question #47589 in Biochemistry for Sherouk Youssef

Question #47589
when preparing the protein standards we used a tube tube without protein but including bradford reagent and an equivalent volume of 0.15 m nacl instead of protein. can we just use plain water to prepare tube t instead?
Expert's answer
At first, I would like to clarify that preparation the protein standards, according to protein assay by Bradford method, covers preparation a serial dilution of the protein sample with known concentration. For this purpose it is usually used  BSA (Bovine Serum Albumin) diluted in 0.15M NaCl from 0 concentration (blank= NaCl only) to the necessary concentration. 0.15 M NaCl corresponds to the  physiological normal state (pH 7.4), that is why it is not advisable to  use water as the conditions for the protein standards should be the same as for the experimental proteins. 

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be first!

Leave a comment

Ask Your question

Submit
Privacy policy Terms and Conditions