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Answer to Question #43333 in Biochemistry for Sumit

Question #43333
Chemical method to determine the number of polypeptide chains in a protein.
Expert's answer
The chemical methodsare usually employed for amino acids analyses, end-group analysis, and quantitative analysisfor specific prosthetic groups. However, the presence of subunits can be established with the help of other methods: gel chromatography, sedimentation velocity, sedimentation equilibrium, and electrophoresis under nondenaturing conditions, followed by a second dimension under denaturing conditions

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