Answer to Question #37402 in Biochemistry for shisha
What is the role of aspartate and glutamate in the catabolism of amino acids
Ammonia created during protein breakdown is very toxic and must find its way to the liver, where is converted to urea for excretion. In most cases, the amino groups, separated from amino acids during early steps in the catabolism of proteins, are transferred to α-ketoglutarate to form glutamate. For transport function, glutamate, critical to intracellular amino group metabolism, is supplanted by L-glutamine. The free ammonia produced in tissues is combined with glutamate to yield glutamine. After transport in the bloodstream, the glutamine enters the liver and converts to glutamate by the enzyme glutaminase. In the muscles glutamate can transfer its α-amino group to pyruvate, a readily available product of muscle glycolysis, thus nitrogen will be transported to the liver as amino group of alanine. In hepatocytes, alanine aminotransferase transfers the amino group from alanine to α-ketoglutarate, forming pyruvate and glutamate. In the liver mitochondria glutamate dehydrogenase liberates the amino group of glutamate as ammonium ion (NH4+) that will be used in urea cycle reactions. On the other hand, glutamate can undergo transamination with oxaloacetate to form aspartate, another nitrogen donor in urea synthesis. As we can see, glutamate and aspartate supply the urea cycle.
Supplying the urea cycle is the special function of glutamate and aspartate in the catabolism of amino acids. But there are functions common for other amino acids. Four- and five-carbon end products of many catabolic processes feed into the Krebs cycle to serve as fuels. Oxaloacetate and α-ketoglutarate are produced from aspartate and glutamate, respectively, when proteins are degraded. Such amino acids as asparagine and arginine can be converted to oxaloacetate and α-ketoglutarate too.
Thank you, but does Aspartate only have one role in catabolism amino acids nitrogen, that it supply the urea cycle?