Answer to Question #337420 in Biochemistry for sana

Question

- Briefly explain why we incubate the purified plasmid with the restriction enzymes, BamHI and XhoI, at exactly 37oC. 

Answer 1

Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

BamHI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

XhoI has 100% activity at 37°C, however there is significant increase of star activity when incubated overnight at 37°C