Answer to Question #255801 in Biochemistry for atenz

Question

A chemical dye absorbs light with wavelength 578 nm.

Using a standard solution of concentration 0.7 mM, the absorption is measured

to be 0.8 units. The absorption for a solution of unknown concentration is then

measured and found to be 0.6 units. What is the concentration? Give the answer to

at least 3 significant figures.

Answer 1

A = εmC +b

578× 0.8× 0.6×0.7= 194.21

= 194