Question #251049

. List and discuss, in your own words, the four key parameters of the Michaelisâ€“Menten Equation. Your response must clearly outline the definition of each of the parameters,and its application to enzyme activity/substrate interaction affinity/catalytic efficiency

Expert's answer

Michaelisâ€“Menten Equation is a model used in enzymes kinetics.

The equation is:"\ud835\udc49 =\\frac{C(Vmax)}{C + Km}"

The four parameters are:

V- The dependent variable.

C- The Independent variable

Vmax is the maximum velocity and may serve as horizontal asymptote.

Km is the ED50 or Michaelis constantÂ .

The Km and Vmax are the two parameters to be calculated.

The rate of enzyme reaction is equal to "\\frac{Vmax \\times\\ concentration}{Km + concentration}"

Therefore, the higher the Km the lower the enzyme reaction as per the above model.

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