Answer to Question #178153 in Biochemistry for sally

Question

Isoelectric focusing is a technique for seperating amino acids.In a mixture of gly-gly-gly and gly-asp-glu, can gly-gly-gly be seperated from gly-asp-glu by isoelectric focusing ? Explain your answer.

Answer 1

Yes , gly-gly-gly can be seperated from gly-asp-glu by isoelectric focusing. This is because the isoelectric point of glutamic acid differ from glycine and aspartic acid. At PH 7 both aspartic and glutamine acid are negatively charged while glycine is positively charged.