For the purposes of the cell adhesion experiment, the cells are then diluted in media such that a 200 µl volume contains 9 x 103 cells. This volume is then added per well for the cell adhesion assay. In total, you will test 40 wells of endothelial cells from each genotype for each experimental condition (with or without TNFα).
Cell adhesion is the process by which cells form contacts with each other or with their substratum through specialized protein complexes. Intercellular adhesion can be mediated by adherens junctions, tight junctions and desmosomes, whereas cells can interact with extracellular matrix molecules through focal adhesions.