Answer to Question #128741 in Biochemistry for AbuBakar

Question

Q3: You have detected a novel uncharacterized RNA which you named Rawal-RNA (It could be mRNA/tRNA/rRNA). When you treat cells with a low dose of alpha-amanitin, levels of Rawal--RNA and GAPDH mRNA DECREASE drastically, whereas tRNA and rRNA levels are unchanged.
a) Which RNA polymerase makes RawalRNA?


b) Explain your reasoning.

Answer 1

a. RNA polymerase II

b. Because Alpha-amanitin has a high affinity for RNA polymerase II in eukaryotic cells