The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS.
Combine the following:
• 80g NaCl
• 2g KCl
• 14.4g Na2HPO4 (dibasic anhydrous)
• 2.4g KH2PO4 (monobasic anhydrous)
• 800mL distilled H2O
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