The surface sedimentation of the sample does sample collection composes within 12 months. The composes specimen are merged and moved to prostrated caballed and contained down to investigation.
Pour plate method enumerations will do the THB population. 1 ml of each inoculated aliquots dilation will be poured into a NA in duplicates by pour plate method. And vaccinated for 23 to 48 hours at a temperature degree of 37celcius and wait for results.
HDB population, the spread of HDB, will be done by inoculating aliquot into sterile BHA by spread plate techniques.
Recognition of HDB and THB culture by enumerating bacteria that were confined and saved to an NA for an enumerated bacteria were isolated and stored in a NA on the support of cell morphology and colony for doing a different kind of biochemical tests. The isolate which will grow in The strains which grow the two agar plate for confirmed as a hydrocarbon.