Both ALDH1 (in cytoplasm) and ALDH2 (in mitochondria) can covert aldehyde to acetate but with different values of Km. According to (Harada et al, 1980), at pH9.5, ALDH1 has Km of 0.1 – 0.4 mM and ALDH2 has Km of 0.01 – 0.02 mM. Which has a higher affinity, and why?
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