Answer to Question #110769 in Biochemistry for rrrrrrr

Question #110769
. A mixture of amino acids consisting of arginine, cysteine, glutamic acid, histidine, leucine, and serine is applied to a strip of paper and subjected to electrophoresis using a buffer at pH 7.5. What are the directions of migration of these amino acids and what are their relative mobilities?
Expert's answer

Electrophoretic mobilities are determined by the average net charge on the amino acid of arginine, cysteine,glutamic acid, histidine, leucine and serine but one is more than 1 pH unit away from any

pKa, and thus will have a single predominant ionic species available by inspection of the titration curve with the exception is cysteine, for which one must use the Henderson-Hasselbalch equation and the pKr

of 8.2 to deduce that about 20% of the cysteine molecules carry a negative charge at pH 7.5

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