Answer to Question #101045 in Biochemistry for Ryan Gamble

Question #101045
NAD+ has negligible absorbable at 340nm. Using a name example, explain how you could use the change in absorbable properties between NADH (molar absorbable 6220M/cm) and NAD+ to measure the rate of a biochemical reaction.
1
Expert's answer
2020-01-07T05:11:25-0500

In spectrophotometric methods, the progress of reaction is monitored by measuring the change in quantity of light the assay reaction absorbs. For example, in simple NAD/NADP .When lactate reacts with NAD+ in the presence of LDH the product is pyruvate and NADH and H+.As the amount of NADH reduces,UV absorbance will decrease


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