Answer to Question #198620 in Biology for hen

Question #198620

The student wanted to study the effect of the different sugars in the rate of growth of a yeast population.

They used to colorimeter to measure the absorbance of a culture of yeast cells.

The absorbance of the yeast culture is proportional to the concentration of yeast cells.

As the yeast is multiplied it was necessary to dilute the sample to obtain a reading on the colorimeter.

Describe how the student could 1cm3 cubed pippets and 10cmmeasuring cylinders to dilute the sample so that it was 10,000 times less concentrated .

Expert's answer

The student should add 9 cm3 of water to four measuring cylinders. Next, the student must take 1 cm3 of the sample via a measuring pipet and add it into the first cylinder. After mixing, the student should take 1 cm3 of the suspension from the first cylinder and add it into the second cylinder. The student must repeat the steps with two other cylinders. As a result, in the last cylinder, the student will get the dilution of the sample 1/10,000.

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