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Answer to Question #63148 in Molecular Biology for ashley

Question #63148
The following is a fragment of the glucocorticoid receptor gene and does not contain the start codon. This gene actually has a mutation at the nucleotide in the 9th fragment (GTC); the wildtype ancestral gene was an A at the T position in the DNA.

1. Transcribe and
2. translate this gene fragment (note: the start codon is NOT included and any codons past a STOP are considered part of the 3’ untranslated region/3’ UTR).
3. Underline the mutation.
4. Identify what type of mutation this is and what it will likely do to the GR protein and to the organism (in terms of its ability to respond to stress).

5’ AGA ATG ACT CTA CCC TGC ATG TAC GTC CAA TGT AAA CAC ATG CTG TAT GTT 3’
Expert's answer
1) 5' AAC AUA CAG CAU GUG UUU ACA UUG _GAC_ GUA CAU GCA GGG UAG AGU CAU UCU 3'
2) Asn Ile Gln His Val Phe Thr Leu _Asp_ Val His Ala Gly
3) mutation Val -> Asp
4) It is missence nonconservation mutation. The mutation changes the polarity of the aminoacid - it incerts highly hydrophilic recidue in the lypophilic region that probably leads to the wrong protein folding. Moreover, the GR protein supposed to bind with lypophilic glucocorticoids, and if this fragment belongs to the binding site, it leads to the protein misfunction.

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