Answer to Question #161583 in Molecular Biology for Virat

Question #161583

The solution of situational problems.

RNA polymerase recognition site -5’ TATATT 3’, -35 nucleotide sequence – 5’ TTGACA 3’

The site of recognition of the repressor protein in the operon (operator) is ATTTTAT, structural genes begin with TAC codons and end with ATC codons, TTTT spacer sites are located between the genes.

a) Using this data, construct a Lac-operon model consisting of three structural genes: the Z- structural gene consists of 5 codons, the Y and A structural genes are 4 codons.

b) Perform a transcription. How much RNA is formed from this site?

c) How many proteins are encoded in this DNA region? Define their structure.


1
Expert's answer
2021-02-15T12:06:48-0500

RNA polymerase recognition site -5’ TATATT 3’, -35 nucleotide sequence – 5’ TTGACA 3’

The site of recognition of the repressor protein in the operon (operator) is ATTTTAT, structural genes begin with TAC codons and end with ATC codons, TTTT spacer sites are located between the genes.

a) Using this data, construct a Lac-operon model consisting of three structural genes: the Z- structural gene consists of 5 codons, the Y and A structural genes are 4 codons.

b) Perform a transcription. How much RNA is formed from this site?

c) How many proteins are encoded in this DNA region? Define their structure.


Answer:

Lac-operon model:

| promoter | operator | | | | | | |

5’-TTGACA-___-TATATT-___-ATTTTAT-___-TTTT-TAC-___-ATC-TTTT-TAC-___-ATC-TTTT-TAC-___-ATC-3’

-35 some -10 recognition lacZ lacY lacA

seq. site of ope-

rator


Only one messenger RNA is formed from this site.


There are 3 proteins that are incoded in this DNA region: β-galactosidase (lacZ),  β-galactoside permease (lacY), β-galactoside transacetylase (lacA).


Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be first!

Leave a comment

Ask Your question

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS