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Answer on Genetics Question for dot

Question #72280
The double-stranded DNA sequence for the coding region of a short peptide is:

1, Which strand of DNA, top or bottom is transcribed by RNA polymerase.Explain.

2, A mutation at the second triplet on the coding strand deletes on of the A-T pairs and creates a frameshift mutation. what is the sequence of the peptide now?

Expert's answer
1. The bottom strand will be transcribed, because its 3'-end has a TAC triplet. It corresponds to AUG start codon in mRNA. The top strand cannot result in mRNA with the start codon.

2. The resulting DNA sequence is
It will result in mRNA with a sequence:

The produced protein will have a sequence:


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