Answer to Question #204600 in Biochemistry for mbali

Question #204600

Discuss the principles of ion exchange and affinity chromatography


1
Expert's answer
2021-06-09T06:21:05-0400

The anion exchange chromatography separates anions. Affinity chromatography is a separation technique based on the specific interaction between an immobilized ligand, and it's binding protein. For example, nickel which has a high affinity to histidine, can be used to separate histidine from a mixture of other protein.


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